5.结论

首先，表面张力测量证明牛精浆具有很好的表面活性，可能与BSP蛋白有关。其次，Langmuir膜法（先前被证明是公牛精子外细胞膜的相关脂质单层模型[35]）有助于表征BSP蛋白对脂质膜的亲和力。BSP蛋白能够到达被磷脂覆盖的牛精子细胞表面，这得益于它们与磷脂酰胆碱的相互作用和自身的表面活性。我们的假设是，由于它们自身的表面活性，它们首先穿透精子的外小叶，然后由于与磷脂酰胆碱的强烈相互作用而留在那里。最后，利用Langmuir膜法对已知的BSP蛋白螯合剂如ldl和脂质体的作用进行了筛选。脂质体与低密度脂蛋白一样有效地阻止BSP蛋白插入磷脂层。低密度脂蛋白和脂质体的比例相同：0.16–0.17 mg磷脂酰胆碱/mg BSP。我们还不知道在冷冻保存的时间尺度上脂质体的非常微弱的插入是否有一些生物学后果。需要进一步研究分析每种BSP蛋白的表面性质及其在膜上的作用。此外，可以测试Langmuir膜，以检测BSP与膜的亲和力随温度的变化[20]。最后，Langmuir膜法是一种有效的筛选BSP蛋白质新螯合剂的方法。该方法可适用于其他动物物种。

致谢

作者感谢IMV Technologies（法国）的财务支持，Gérard Chatagnon负责精浆蛋白质定量，Véronique Solé负责凝胶电泳，Maureen Collobert负责英语校正，Alain Sire和Patrice Papineau负责手套箱的构思和实现，允许在受控大气中使用朗缪尔槽。

附录A.补充数据

与本文相关的补充数据可在在线版本中找到，网址为http://dx.doi.org/10.1016/j.colsurfb.2015.11.34

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