结论

在本研究中，从被石油烃污染的海洋环境中获得了 18 株耐寒且能够产生生物 SAC 的分离株。 分离物是假单胞菌属、假交替单胞菌属、红球菌属、链球菌属、Cobetia、Glaciecola、Marinomonas、Serratia 和 Psychromonas 的成员。 其中，红球菌属。 LF-13 和红球菌属。 在油性底物（如煤油、正十六烷或菜籽油）存在的情况下，LF-22 能够显着降低培养基的表面张力。 两种菌株中的生物表面活性剂合成不一定与生长相关，这表明静息细胞可用于从两种红球菌菌株中生产生物表面活性剂。 从红球菌属中提取的生物表面活性剂。 菌株 LF-22 能够提高正十六烷在 13°C 的生物降解率。 应纯化这些分离物中的生物表面活性剂，以进一步阐明其化学结构和特性，并研究其在溢油生物修复和其他行业中的应用。

披露声明

作者没有报告潜在的利益冲突。

资金

本文中描述的工作得到了挪威研究委员会和 ENI Norge [项目编号 195160] 的资助以及 MABIT 计划 [项目编号 BS0052] 的资助。

参考

[1] Rodrigues L, Banat IM, Teixeira J, Oliveira R. Biosurfactants: potential applications in medicine. J Antimicrob Chemother. 2006;57:609–618.

[2] Muthusamy K, Gopalakrishnan S, Ravi TK, Sivachidambaram P. Biosurfactants: properties, commercial production and application. Curr Sci. 2008;94:736–747.

[3] Pattanathu KSM, Gakpe E. Production, characterization and applications of biosurfactants-review. Biotechnology. 2008;7:360–370.

[4] Paramaporn C, Phonnok S, Durand A, Marie E, Thanomsub BW. Bioproduction and anticancer activity of biosurfactant produced by the dematiaceous fungus Exophiala dermatitidis SK80. J Microbiol Biotechnol. 2010;20:1664–1671.

[5] Kosaric N. Biosurfactants and their application for soil bioremediation. Food Technol Biotechnol. 2001;39:295–304.

[6] Mulligan CN. Environmental applications for biosurfactants. Environ Pollut. 2005;133:183–198.

[7] Damasceno FRC, Freire DMG, Cammarota MC. Assessing a mixture of biosufactant and enzyme pools in the anaerobic biological treatment of wastewater with high-fat content. Environ Technol. 2014;35:2035–2045.

[8] Cheng KY, Zhao ZY,Wong JWC. Solubilization and desorption of PAHs in soil aqueous system by biosurfactants produced from Pseudomonas aeruginosa P-CG3 under thermophilic condition. Environ Technol. 2004;25:1159–1165.

[9] Whyte LG, Slagman SJ, Pietrantonio F, Bourbonnière L, Koval SF, Lawrence JR, Inniss WE, Greer CW. Physiological adaptations involved in alkane assimilation at low temperatures by Rhodococcussp. strain Q15. Appl Environ Microbiol. 1999;65:2961–2968.

[10] Yakimov MM, Giuliano L, Bruni V, Scarfı S, Golyshin PN. Characterization of Antarctic hydrocarbon-degrading bacteria capable of producing bioemulsifiers. New Microbiol. 1999;22:249–259.

[11] Bushnell LD, Haas HF. The utilization of certain hydrocarbons by microorganisms. J Bacteriol. 1941;41:653–673.

[12] Chen CY, Baker SC, Darton RC. The application of a high throughput analysis method for screening of potential biosurfactants from natural sources. J Microbiol Methods. 2007;70:503–510.

[13] Walter V, Syldatk C, Hausmann R. Biosurfactants. New York: Springer; 2010. Chapter 1, Screening concepts for the isolation of biosurfactant producing microorganisms; p. 1–13.

[14] Batista S, Mounteer A, Amorim F, Tótolaa MR. Isolation and characterization of biosurfactant/bioemulsifier producing bacteria from petroleum contaminated sites. Bioresour Technol. 2006;97:868–875.

[15] Tadros T. Applied surfactants: principle and applications. Weinheim: Wiley VCH; 2005. Adsorption of surfactants at the air/liquid and liquid/liquid interface; p. 81–82.

[16] Kuyukina MS, Ivshina IB, Philip JC, Christofi N, Dunbar SAE, Ritchkova MI. Recovery of Rhodococcus biosurfactants using methyl tertiary-butyl ether extraction. J Microbiol Methods. 2001;46:149–156.

[17] Zhang C. Fundamentals of environmental sampling and analysis. Hoboken, NJ: Wiley; 2007. Chapter 6, Common operations and wet chemical methods in environmental laboratories; p. 148–149.

[18] Olivera NL, Commendatore MG, Delgado O, Esteves JL. Microbial characterization and hydrocarbon biodegradation potential of natural bilge waste microflora. J Ind Microbiol Biotechnol. 2003;30:542–548.

[19] Willumsen PAE, Karlson U. Screening of bacteria, isolated from PAH-contaminated soils for production of biosurfactants and bioemulsifiers. Biodegradation. 1997;7:415–423.

[20] Sapute SK, Bhawsar BD, Dhakephalkar PK, Chopade BA. Assessment of different screening methods for selecting biosurfactant producing marine bacteria. Indian J Marine Sci. 2008;37:243–250.

[21] Yakimov MM, Gentile G, Bruni V, Cappello S, D'Auria G, Golyshin PN, Giuliano L. Crude oil-induced structural shift of coastal bacterial communities of rod bay (Terra Nova Bay, Ross Sea, Antarctica) and characterization of cultured cold-adapted hydrocarbonoclastic bacteria. FEMS Microbiol Ecol. 2009;49:419–432.

[22] Gerdes B, Brinkmeyer R, Dieckmenn G, Helmke E. Influence of crude oil on changes of bacterial communities in Arctic sea-ice. FEMS Microbiology Ecology. 2005;53:129–139.

[23] Brakstad OG, Bonaunet K. Biodegradation of petroleum hydrocarbons in seawater at low temperatures (0–5°C) and bacterial communities associated with degradation. Biodegradation. 2006;7:71–82.

[24] Margesin R. Alpine microorganisms: useful tools for lowtemperature bioremediation. J Microbiol. 2007;45:281–285.

[25] Grossman M, Prince R, Garret R, Garrett K, Bare R, Lee K, Sergy G, Owens E, Guénette C. Microbial diversity in oiled and unoiled shoreline sediments in the Norwegian Arctic. In: Bell CR, Brylinsky M, Johnson-Green P, editors. The 8th international symposium on microbial ecology. Proceedings; 1998 Aug 9–14; Halifax, NS (Canada). [26] Deppe U, Richnow HH, Michaelis W, Antranikian G. Degradation of crude oil by an Arctic microbial consortium. Extremophiles. 2005;9:461–470.

[27] Brakstad OG, Nonstad I, Faksness LG, Brandvik PJ. Responses of microbial communities in Arctic sea ice after contamination by crude petroleum oil. Microb Ecol. 2008;55:540–552.

[28] Røberg S, Østerhus JI, Landfald B. Dynamics of bacterial community exposed to hydrocarbons and oleophilic fertilizer in high-Arctic intertidal beach. Polar Biol. 2011;34:1455– 1465.

[29] Déziel E, Lépine F, Milot S, Villemur R. rhlA is required for the production of a novel biosurfactant promoting swarming motility in Pseudomonas aeruginosa: 3-(3- hydroyalkanoyloxy) alkanoic acids (HAAs), the precursors of rhamnolipids. Microbiology. 2003;149:2005–2013. [30] Benincasa M, Abalos A, Oliveira I, Manresa A. Chemical structure, surface properties and biological activities of the biosurfactant produced by Pseudomonas aeruginosa LBI from soap stock. Antonie van Leeuwenhoek. 2004;85:1–8.

[31] Neu TR, Haertner T, Poralla K. Surface active properties of viscosin: a peptidolipid antibiotic. Appl Microbiol Biotechnol. 1990;32:518–520.

[32] Pedras MSC, Ismail N, Quail JW, Boyetchko SM. Structure, chemistry, and biological activity of pseudophomins A and B, new cyclic lipodepsipeptides isolated from the biocontrol bacterium Pseudomonas fluorescens. Photochemistry. 2003;62:1105–1114.

[33] Kuiper I, Lagendijk EL, Pickford R, Derrick JP, Lamers GEM, Thomas-Oates JE. Characterization of two Pseudomonas putida lipopeptide biosurfactants, putisolvin I and II, which inhibit biofilm formation and break down existing biofilms. Mol Microbiol. 2004;51:97–113.

[34] Anu-Appaiah KA, Karanth NGK. Insecticide specific emulsi- fier production by hexachlorocyclohexane utilizing Pseudomonas tralucida Ptm strain. Biotechnol Lett. 1991;13:371–374.

[35] Bonilla M, Olivaro C, Corona M, Vazquez A, Soubes M. Production and characterization of a new bioemulsifier from Pseudomonas putida ML2. J Appl Microbiol. 2005;98:456–463.

[36] Uchida Y, Tsuchiya R, Chino M, Hirano J, Tabuchi T. Extracellular accumulation of mono- and di-succinoyl trehalose lipids by a strain of Rhodococcus erythropolis grown on n-alkanes. Agric Biol Chem. 1989;53:757–763.

[37] Lang S, Philp CJ. Surface active lipids in Rhodococci. Antonie van Leeuwenhoek. 1998;74:59–70. [38] Peng F, Liu Z, Wang L, Shao Z. An oil-degrading bacterium: Rhodococcus erythropolis strain 3C-9 and its biosurfactants. J Appl Microbiol. 2007;102:1603–1611.

[39] Rougeaux H, Quezennec J, Carlson RW, Kervarec N, Pichon R, Talaga P. Structural determination of the exopolysaccharide of Pseudoalteromonas strain HYD 721 isolated from a deepsea hydrothermal vent. Carbohydr Res. 1999;315:273–285.

[40] Mancuso-Nichols C, Garon S, Bowman JP, Raguénès G, Guézennec J. Production of exopolysaccharides by Antarctic marine bacterial isolates. J Appl Microbiol. 2004;96:1057–1066.

[41] Mancuso-Nichols C, Bowman JP, Guezennec J. Effects of incubation temperature on growth and production of exopolysaccharides by an Antarctic sea ice bacterium grown in batch culture. Appl Environ Microbiol. 2005;71:3519–3523.

[42] Gutiérrez T, Shimmield T, Haidon C. Emulsifying and metal ion binding activity of a glycoprotein exopolymer produced by Pseudoalteromonas sp. strain TG12. Appl Environ Microbiol. 2008;74:4867–4876.

[43] Matsuyama H, Hirabayashi T, Kasahara H, Minami H, Hoshino T, Yumoto I. Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol. 2006;56:2883–2886.

[44] Fiebig R, Schulze D, Chung JC, Lee ST. Biodegradation of biphenyls (PCBs) in the presence of a bioemulsifier produced on sunflower oil. Biodegradation. 1997;8:67–75.

[45] Cameotra SS, Makkar RS. Synthesis of biosurfactants in extreme conditions. Appl Microbiol Biotechnol. 1998;50:520–529.

[46] Kim JS, Powalla M, Lang S, Wagner F, Lunsdorf H, Wray V. Microbial glycolipid production under nitrogen limitation and resting cell conditions. J Biotechnol. 1990;13:257–266.

[47] Kitamoto D, Fuzishiro T, Yanagishita H, Nakane T, Nakahara T. Production of mannosylerythritol lipids as biosurfactants by resting cells of Candida Antarctic. Biotechnol Lett. 1992;14:305–310.

海洋细菌中生物表面活性物质——摘要、介绍

海洋细菌中生物表面活性物质——材料和方法

海洋细菌中生物表面活性物质——结果和讨论

海洋细菌中生物表面活性物质——结论、致谢！