结论

首先，表面张力测量证明牛精浆具有非常好的表面活性，这可能与 BSP 蛋白。 二、朗缪尔薄膜法 先前被证明是相关的脂质单层模型 公牛精子的外细胞膜 [35]，有助于表征 BSP 蛋白对脂质膜的亲和力。 BSP 蛋白是 能够到达被磷脂覆盖的牛精子细胞表面，这要归功于它们与磷脂酰胆碱和 它们自身的表面活性。 我们的假设是，由于它们自己的表面，它们首先穿透精子的外叶 活动然后他们留在那里因为与 磷脂酰胆碱。 最后，Langmuir 薄膜法也得到了 用于筛选已知的 BSP 蛋白螯合剂（如 LDL 和脂质体）的作用。 脂质体被证明是 与 LDL 一样有效地防止 BSP 蛋白插入 磷脂层。 发现 LDL 和 脂质体：0.16–0.17 mg 磷脂酰胆碱/mg BSP。 我们不 还知道脂质体在 冷冻保存的时间尺度有一些生物学后果。 需要进一步的研究来分析每个的表面特性 BSP 蛋白及其对膜的作用。 此外，可以测试朗缪尔薄膜以检测 BSP 对膜的亲和力随温度的变化 [20]。 最后，朗缪尔薄膜法是一种 用于鉴定新的螯合剂的强大筛选方法 BSP 蛋白质。 这种方法可以适用于其他动物物种。

致谢

作者要感谢 IMV Technologies（法国） 其财政支持，Gérard Chatagnon 的量化 精浆中的蛋白质，凝胶电泳的 Véronique Solé，英语校正的 Maureen Collobert 和 Alain Sire 和 Patrice Papineau 的概念和实现 手套箱允许在受控的情况下使用 Langmuir 槽进行工作 大气层。

附录 A. 补充数据

与本文相关的补充数据可以在 在线版本，见 http://dx.doi.org/10.1016/j.colsurfb.2015.11。 034.

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应用不同组装的磷脂酰胆碱对牛精浆蛋白的隔离——摘要、简介

应用不同组装的磷脂酰胆碱对牛精浆蛋白的隔离——材料与方法

应用不同组装的磷脂酰胆碱对牛精浆蛋白的隔离——结果与讨论

应用不同组装的磷脂酰胆碱对牛精浆蛋白的隔离——结论、致谢！